Post by Melanie on Jan 4, 2015 21:10:40 GMT 1
Thelotrema philippinum Rivas Plata, Sipman & Lücking, sp. nov. (Fig. 1F)
Mycobank #807529
Differing from Thelotrema suecicum in the stictic acid chemistry.
Type:—PHILIPPINES. Sorsogon: Luzon Island, Irosin; on branchlets of Vavaea luzonense; April 1916, Elmer 16476 (holotype: B!; isotype: F!).
Thallus corticolous, epiperidermal, up to c. 5 cm diam., continuous; surface uneven to verrucose, yellowish grey; prothallus absent. Thallus in section 50–60 μm thick, with loose cortex, 10–20 μm thick, photobiont layer, 20–30 μm thick, and medulla mostly developed in the verrucae, 10–30 μm thick, filled with clusters of calcium oxalate crystals. Photobiont Trentepohlia; cells rounded to irregular in outline, in irregular groups, olive-green, 8–11 × 5–10 μm. Ascomata rounded, prominent, with complete thalline margin, 0.5–0.6 mm diam., 0.15–0.2 mm high; disc covered by 0.1–0.2 mm wide pore; proper margin distinct, entire, forming a prominent rim around the disc and separated from the thalline margin by a split; thalline margin thick, entire, smooth to uneven, light yellowish grey. Excipulum entire, yellowish, 20–30 μm wide, paraplectenchymatous, separated from thalline margin by a split; laterally covered by algiferous, corticate thallus including orange-brown periderm layer; columella absent (degenerated hymenia may resemble a pseudocolumella; hypothecium prosoplectenchymatous, 10–20 μm high, hyaline; hymenium 100–120 μm high, hyaline, clear; epithecium 5–10 μm high, grey, granular. Paraphyses unbranched, apically smooth; periphysoids present, 20–30 μm long; asci fusiform, 100–120 × 15–18 μm. Ascospores (4–)8 per ascus, oblong with tapering distal end, 11–15-septate, 40–55 × 10–12 μm, 3.5–4.5 times as long as wide, hyaline, distoseptate with lens-shaped lumina, thick-walled, I+ violet-blue.
Secondary chemistry:—Stictic and constictic acids; medulla P+ orange, microscopic section with K+ persistently yellow efflux.
Distribution and ecology:—Only known from historic collections from a probably vanished rain forest area on the island of Luzon; possibly extinct.
Remarks:—This new species is morphologically identical with Thelotrema suecicum (H. Magn.) James (1977: 186) and has typical Thelotrema-type thallus and ascomata, but differs chiefly in the stictic acid chemistry. Also, the ascospores, while displaying the same morphology and iodine reaction as in T. suecicum, are slightly larger in size (30–40 μm long in T. suecicum).
Five new thelotremoid Graphidaceae from the Philippines
EIMY RIVAS PLATA, HARRIE J. M. SIPMAN & ROBERT LÜCKING
www.mapress.com/phytotaxa/content/2014/f/p00189p288f.pdf
Mycobank #807529
Differing from Thelotrema suecicum in the stictic acid chemistry.
Type:—PHILIPPINES. Sorsogon: Luzon Island, Irosin; on branchlets of Vavaea luzonense; April 1916, Elmer 16476 (holotype: B!; isotype: F!).
Thallus corticolous, epiperidermal, up to c. 5 cm diam., continuous; surface uneven to verrucose, yellowish grey; prothallus absent. Thallus in section 50–60 μm thick, with loose cortex, 10–20 μm thick, photobiont layer, 20–30 μm thick, and medulla mostly developed in the verrucae, 10–30 μm thick, filled with clusters of calcium oxalate crystals. Photobiont Trentepohlia; cells rounded to irregular in outline, in irregular groups, olive-green, 8–11 × 5–10 μm. Ascomata rounded, prominent, with complete thalline margin, 0.5–0.6 mm diam., 0.15–0.2 mm high; disc covered by 0.1–0.2 mm wide pore; proper margin distinct, entire, forming a prominent rim around the disc and separated from the thalline margin by a split; thalline margin thick, entire, smooth to uneven, light yellowish grey. Excipulum entire, yellowish, 20–30 μm wide, paraplectenchymatous, separated from thalline margin by a split; laterally covered by algiferous, corticate thallus including orange-brown periderm layer; columella absent (degenerated hymenia may resemble a pseudocolumella; hypothecium prosoplectenchymatous, 10–20 μm high, hyaline; hymenium 100–120 μm high, hyaline, clear; epithecium 5–10 μm high, grey, granular. Paraphyses unbranched, apically smooth; periphysoids present, 20–30 μm long; asci fusiform, 100–120 × 15–18 μm. Ascospores (4–)8 per ascus, oblong with tapering distal end, 11–15-septate, 40–55 × 10–12 μm, 3.5–4.5 times as long as wide, hyaline, distoseptate with lens-shaped lumina, thick-walled, I+ violet-blue.
Secondary chemistry:—Stictic and constictic acids; medulla P+ orange, microscopic section with K+ persistently yellow efflux.
Distribution and ecology:—Only known from historic collections from a probably vanished rain forest area on the island of Luzon; possibly extinct.
Remarks:—This new species is morphologically identical with Thelotrema suecicum (H. Magn.) James (1977: 186) and has typical Thelotrema-type thallus and ascomata, but differs chiefly in the stictic acid chemistry. Also, the ascospores, while displaying the same morphology and iodine reaction as in T. suecicum, are slightly larger in size (30–40 μm long in T. suecicum).
Five new thelotremoid Graphidaceae from the Philippines
EIMY RIVAS PLATA, HARRIE J. M. SIPMAN & ROBERT LÜCKING
www.mapress.com/phytotaxa/content/2014/f/p00189p288f.pdf
